Santa Fe Trail is a 1940 American Western film directed by Michael Curtiz and starring Errol Flynn as J. E. B. "Jeb" Stuart, Olivia de Havilland, Raymond Massey as John Brown, Ronald Reagan as George Armstrong Custer and Alan Hale. Written by Robert Buckner, the film is critical of the abolitionist John Brown and his controversial campaign against slavery before the American Civil War. In a subplot, Jeb Stuart and George Armstrong Custer—who are depicted as friends from the same West Point graduating class—compete for the hand of Kit Carson Holliday.

The film ranked among the higher grossing films of the year, and the seventh Flynn–de Havilland collaboration. Its content has little relevance to the actual Santa Fe Trail.