Totally Spies! The Movie (French: Totally Spies! Le film) is a 2009 animated spy-action comedy film directed by Pascal Jardin and written by Robert and Michelle Lamoreaux. A French-Italian co-production, it is an adaptation of the TF1's animated television series Totally Spies! created by Vincent Chalvon-Demersay and David Michel.

The film is a prequel to the television series and covers how the girls first met and shows how they became spies for the World Organization of Human Protection (WOOHP). It stars the voices of the original French cast of the television series, and also features the voice of German fashion designer Karl Lagerfeld as the main antagonist. It is the first theatrical film based on a TF1's TFOU animated series.

Totally Spies! The Movie premiered at the Grand Rex on 28 June 2009, and was released in France on 22 July 2009 by Mars Distribution. It received mixed reviews from critics. Internationally, it made its debut direct-to-television on Cartoon Network.