With Buffalo Bill on the U.P. Trail, alternately called Buffalo Bill on the U.P. Trail, is a 1926 American silent historical Western film starring Roy Stewart as Buffalo Bill Cody. It was directed by Frank Mattison and produced by Anthony J. Xydias.

This film is available from the Prelinger Archives, San Francisco and the Grapevine video, Phoenix, and is preserved at the Library of Congress.

Several members of the cast and crew of this film later worked on General Custer at the Little Big Horn (1926).