In Old Santa Fe is a 1934 American Western film directed by David Howard, starring Ken Maynard, George "Gabby" Hayes and Evalyn Knapp and featuring the first screen appearance of Gene Autry, singing a bluegrass rendition of "Wyoming Waltz" accompanied by his own acoustic guitar with Smiley Burnette on accordion. Autry and Burnette were uncredited, but the scene served as a screen test for the duo for subsequent singing cowboy films, beginning with The Phantom Empire (1935), in which Autry had his first leading role.

Based on a story by Wallace MacDonald and John Rathmell, the film is about a cowboy who loses his horse in a rigged horse race and gets framed for the murder of a stagecoach driver. The film was shot on location in Kentucky and Keystone Studios in California.