G.I. Joe: The Rise of Cobra is a 2009 American military science fiction action film based on the G.I. Joe toy line. It is the first installment in the G.I. Joe film series. Directed by Stephen Sommers from a screenplay by Stuart Beattie, David Elliot, and Paul Lovett, the film features an ensemble cast based on the various characters of the toy line. The story follows two American soldiers, Duke and Ripcord, who join the G.I. Joe Team after being attacked by Military Armaments Research Syndicate (M.A.R.S.) troops.

After leaked drafts of the script were criticized by fans, Larry Hama, writer of the comic book series G.I. Joe: A Real American Hero, was hired as creative consultant, and rewrites were made. Filming took place in Downey, California and Prague's Barrandov Studios, and six companies handled the visual effects.

G.I. Joe: The Rise of Cobra premiered at the Andrews Air Force Base on July 31, 2009, and was released in the United States on August 7, by Paramount Pictures, following an extensive marketing campaign focused on the Mid-American public. Despite mostly negative reviews from critics, the film grossed $302.5 million worldwide against a $175 million budget, making it a box-office success.

A sequel, titled G.I. Joe: Retaliation, was released in 2013.