A Gruesome Twosome is a 1945 Warner Bros. Merrie Melodies cartoon directed by Bob Clampett. The short was released on June 9, 1945, and stars Tweety.

This is the last Tweety film directed by Clampett, following 1942's A Tale of Two Kitties and 1944's Birdy and the Beast, and the last one before he is permanently paired with Sylvester. One of the cats in this cartoon is a caricature of the comedian Jimmy Durante.

In the film, a female cat attempts to end a love triangle involving her and her two suitors, by proclaiming that she will choose the male who can bring her a bird as an offering. The two rival males choose Tweety as their prey, who persistently outwits them.