The 2016 Fastlane was the second Fastlane professional wrestling pay-per-view (PPV) and livestreaming event produced by WWE. It took place on February 21, 2016, at Quicken Loans Arena in Cleveland, Ohio. This was the last Fastlane to be held before the reintroduction of the brand extension in July.

Eight matches were contested at the event, including one on the Kickoff pre-show. In the main event, Roman Reigns defeated Brock Lesnar and Dean Ambrose in a triple threat match to become the #1 contender for the WWE World Heavyweight Championship to face Triple H at WrestleMania 32. In other prominent matches, AJ Styles defeated Chris Jericho, and Charlotte defeated Brie Bella to retain the WWE Divas Championship.