The Mystery of the Yellow Room is a 1919 American crime drama film made by the Mayflower Photoplay Company and distributed through Realart Pictures Corporation. Émile Chautard served as a producer.

The Mystery of the Yellow Room (in French Le mystère de la chambre jaune) was originally a novel by Gaston Leroux, one of the first locked room mystery novels. It was first published in France in the periodical L'Illustration from September 1907 to November 1907, then in its own right as a book in 1908.

The film, which itself is a remake of Chautard's "Le Mystère de la chambre jaune" (1913), was remade in 1930, 1949 and in 2003.