The 2010 Night of Champions was the fourth annual Night of Champions professional wrestling pay-per-view (PPV) event produced by World Wrestling Entertainment (WWE). It was held for wrestlers from the promotion's Raw and SmackDown brand divisions. The event took place on September 19, 2010, at the Allstate Arena in the Chicago suburb of Rosemont, Illinois. It was the final Night of Champions held during the first brand split, which ended in August 2011, but was reinstated in July 2016. The concept of the show was that every championship in the company at the time was defended. It was also the first Night of Champions event to feature a non-title match, where Big Show defeated CM Punk.

Seven matches took place at the event. There were two main events: one from Raw and one from SmackDown. In the Raw main event, Randy Orton defeated defending champion Sheamus, John Cena, Wade Barrett, Chris Jericho, and Edge in a six-man elimination challenge match to win the WWE Championship and in the SmackDown main event, Kane defeated The Undertaker in a No Holds Barred match to retain the World Heavyweight Championship. Other prominent matches included Dolph Ziggler retaining the WWE Intercontinental Championship against Kofi Kingston, and Daniel Bryan defeated The Miz to win the WWE United States Championship.

The event had 165,000 buys, down on the 2009's Night of Champions figure of 267,000 buys.