Gildersleeve's Ghost is a 1944 American fantasy comedy film directed by Gordon Douglas from an original screenplay by Robert E. Kent. It is the fourth and final film in the Gildersleeve's series, all of which were produced and distributed by RKO Radio Pictures, based on the popular NBC radio program, The Great Gildersleeve, created by Leonard L. Levinson and itself a spin-off of Fibber McGee and Molly. Released on September 6, 1944, the film stars Harold Peary, Marion Martin, Richard LeGrand, Amelita Ward, Freddie Mercer, and Margie Stewart.

The film initially focuses on elections for the position of police commissioner, with Gildersleeve as a candidate. He tries to boost his chances by investigating a series of questionable scientific experiments. But Gildersleeve's sanity is questioned when he meets the ghosts of his own ancestors, an invisible woman, and a gorilla.