Symphony Hour is a 1942 American animated short film produced by Walt Disney Productions and released by RKO Radio Pictures. The cartoon depicts Mickey Mouse conducting a symphony orchestra sponsored by Pete. The film was directed by Riley Thomson and features music adapted from the "Light Cavalry Overture" by Franz von Suppé. The voice cast includes Walt Disney as Mickey, Billy Bletcher as Pete (as Sylvester Macaroni), and John McLeish as a radio announcer. It was the 117th short in the Mickey Mouse film series to be released, and the second for that year.

The film marked the last theatrical appearance of Horace Horsecollar, Clarabelle Cow, and Clara Cluck for over 40 years, finally reappearing in Mickey's Christmas Carol (1983). Symphony Hour is also the last time that Mickey appeared with either Donald Duck or Goofy in a theatrical film for the same length of time.

Symphony Hour bears similarities with the 1935 film The Band Concert. Leonard Maltin called this short a "Spike Jones version of The Band Concert".

The soundtrack for the "ruined" version of the Light Cavalry Overture was used in the October 22, 1956 episode of The Mickey Mouse Club.