Scooby-Doo! Mystery Incorporated (also known as Scooby-Doo! Mystery, Inc. or simply Mystery Incorporated) is an American animated horror comedy television series serves as the eleventh incarnation of the Scooby-Doo media franchise created by Hanna-Barbera, as well as the first that was not originally run on Saturday mornings. The series is produced by Warner Bros. Animation for Cartoon Network UK and premiered in the United States on Cartoon Network on April 5, 2010, with the next twelve episodes continuing, and the first episode re-airing, on July 12, 2010. The series concluded on April 5, 2013, after two seasons and fifty-two episodes.

Mystery Incorporated returns to the early days of Scooby and the gang, when they are still solving mysteries in their home town, though it makes multiple references to previous incarnations of the franchise. The series takes a tongue-in-cheek approach to the classic Scooby-Doo formula, with increasingly outlandish technology, skills, and scenarios making up each villain's story, and a different spin on the famous "meddling kids" quote at the end of every episode. Contrasting sharply with this, however, are two elements that have never been used in a Scooby-Doo series before: a serial format with an ongoing story arc featuring many dark plot elements that are treated with near-total seriousness, and ongoing relationship drama among the characters. Furthermore, it is also the first series in the franchise to make use of real ghosts and monsters since The 13 Ghosts of Scooby-Doo.

The series pays homage to the horror genre, drawing on many works from film, television and literature in both parodic and serious ways, from horror film classics like A Nightmare on Elm Street, modern films such as Saw, television series Twin Peaks, and the works of H. P. Lovecraft, alongside the classic monster horror films shown in previous series. In particular, in the second season, the central story arc evolves to heavily feature the use of Babylonian mythology, exploring the Anunnaki, the Babylonian and modern pseudo-scientific concepts of Nibiru, and the writings of Zecharia Sitchin. Other Hanna-Barbera characters occasionally guest-star, such as Captain Caveman, Jabberjaw, Speed Buggy, The Funky Phantom, and Blue Falcon and Dynomutt.

As was the case with the previous three installments in the franchise, Mystery Incorporated redesigns the main characters, this time into a retro look that returns them to their original 1969 outfits, with some small changes (such as Velma now wearing bows in her hair). The series is also the animated debut of Matthew Lillard as the voice of Shaggy, after he portrayed the character in two live-action films, Scooby-Doo (2002) and Scooby-Doo 2: Monsters Unleashed (2004). Casey Kasem, the original voice of Shaggy, voiced Shaggy's father in five episodes, albeit uncredited; this would be his last voice-acting role before his death. Linda Cardellini, who played Velma in the live-action movies, voiced Hot Dog Water, a recurring character in the series. The show also brought back characters seen in previous Scooby-Doo series such as the eco-goth rock band The Hex Girls and Vincent Van Ghoul from The 13 Ghosts of Scooby-Doo, though his character is portrayed as a direct homage to Vincent Price, being a famous horror film actor, rather than an actual warlock.