Totally Spies! is a French anime-influenced animated spy-fi series created by Vincent Chalvon-Demersay and David Michel mainly produced by French company Zodiak Kids & Family France, with seasons three to five being co-produced with Canadian company Image Entertainment Corporation. It focuses on three teenage girls from Beverly Hills, California, who work as undercover agents for the World Organization of Human Protection (WOOHP).

Prior to its debut in France on 3 April 2002 on TF1's TFOU block, the series was first shown in the United States on 3 November 2001 on ABC Family before moving to Cartoon Network, and later to Universal Kids for the sixth season. It premiered in Canada on Teletoon on 2 September 2002 for the English version, and on Télétoon for the French version. During its original run, the show spanned five seasons, with 130 episodes and several specials. In 2011, the series was moved to Gulli, with the release of the sixth season in 2013. Following a second hiatus that lasted ten years, a seventh season was premiered in France via Gulli on 12 May 2024, and internationally through both Cartoon Network and Discovery Kids on 4 January 2025; the series is renewed for an eighth season.

Totally Spies developed a cult following worldwide. The series also spawned a media franchise with several products tied to it, including various comic books, novels and video games. A prequel film, Totally Spies! The Movie, was released theatrically in 2009 in France. A spin-off series titled The Amazing Spiez! premiered in 2009 and ran for two seasons.