Johnny Test is an animated television series created by Scott Fellows, originally produced in the United States by Warner Bros. Animation and later produced in Canada by Cookie Jar Entertainment. It premiered on Kids' WB on September 17, 2005, which continued to air the series through its second and third seasons. The series aired on Cartoon Network on January 7, 2008, in the United States, and ended its run on December 25, 2014. In Canada, the show premiered on Teletoon on September 3, 2006.

The series revolves around the adventures of the titular character, Johnny Test, an 11-year-old suburban boy who lives with his parents, his "super-genius" 13-year-old twin sisters, Susan and Mary, who are scientists and best friends with each other, and a talking dog named Dukey. They reside in the fictional town of Porkbelly in North America. Johnny is often used as a test subject for his genius twin sisters' inventions and experiments, ranging from gadgets to superpowers. Their experiments often cause problems that he must resolve, and he must sometimes fight villains in the process. He occasionally saves the world with his sisters' inventions.

In 2013, Teletoon originally announced that Johnny Test was going to receive a seventh season, but the show was cancelled and the planned season was never created. In spite of this, WildBrain confirmed that a revival series has been picked up by Netflix for two seasons and a 66-minute interactive special on May 6, 2020. The revival series was released in 2021 with Fellows returning as showrunner and executive producer.