Night at the Museum is a 2006 American fantasy comedy film directed by Shawn Levy and written by Robert Ben Garant and Thomas Lennon. It is based on the 1993 children's book of the same name by Croatian illustrator Milan Trenc. The film had an ensemble cast of Ben Stiller in the lead role, Carla Gugino, Dick Van Dyke, Mickey Rooney, Bill Cobbs, and Robin Williams. It tells the story of a divorced father who applies for a job as a night watchman at New York City's American Museum of Natural History and subsequently discovers that the exhibits come to life at night, animated by a magical Egyptian artifact. 20th Century Fox released the film on December 22, 2006, and it grossed $574.5 million worldwide, becoming the fifth highest-grossing film of 2006, but received mixed reviews from critics.

Two sequels were released, Night at the Museum: Battle of the Smithsonian in 2009, and Night at the Museum: Secret of the Tomb in 2014. An animated sequel, Night at the Museum: Kahmunrah Rises Again, was released on December 9, 2022, for the streaming service Disney+.