Bio-Dome is a 1996 American comedy film directed by Jason Bloom. It was produced by Motion Picture Corporation of America on a budget of $8.5 million and was distributed theatrically by Metro-Goldwyn-Mayer Pictures.

The film was inspired by the real life project Biosphere 2. The plot revolves around two clumsy, dim-witted slackers who, while on a road trip, look for a bathroom in what they believe is a shopping mall. The shopping mall turns out to be a "bio-dome", a form of closed ecological system, in which five scientists are about to be hermetically sealed for a year. The film has themes of environmentalism, combined with drug use, sexual innuendo, and toilet humor.

The film stars Stephen Baldwin and Pauly Shore, and has cameo appearances by celebrities such as Roger Clinton and Patricia Hearst. Jack Black and Kyle Gass first came to global attention in Bio-Dome, performing together as Tenacious D on-screen for the first time.

The film was financed by a loan from Coutts & Co. to the Motion Picture Corporation of America. It film grossed $13 million at the box office in North America. Bio-Dome was panned by critics, gaining a record low score of 1/100 on Metacritic. It is often considered to be one of the worst films ever made.

In 2013, Stephen Baldwin said he was in talks with Pauly Shore about making a sequel to the film revolving around the children of Bud and Doyle, their characters.