Spy Kids 3-D: Game Over (also known as Spy Kids 3: Game Over) is a 2003 American spy action comedy film co-produced, written, shot, edited, composed, and directed by Robert Rodriguez. It is the sequel to Spy Kids 2: The Island of Lost Dreams (2002) and the third installment in the Spy Kids film series. The film stars Antonio Banderas, Carla Gugino, Alexa Vega, Daryl Sabara, Ricardo Montalbán, Holland Taylor, Mike Judge, Cheech Marin, and Sylvester Stallone.

Spy Kids 3-D: Game Over premiered at the Paramount Theatre in Austin, Texas on July 13, 2003, and was released in the United States on July 25, by Dimension Films. Despite mixed reviews from critics, the film grossed over $197 million worldwide against a production budget of $38 million, becoming the highest-grossing film in the series.

Though this was initially intended to be the final installment in the Spy Kids film series, it was eventually followed by a fourth film, Spy Kids: All the Time in the World, in 2011, and a fifth, Spy Kids: Armageddon, in 2023.