The 2007 One Night Stand was the third annual One Night Stand professional wrestling pay-per-view (PPV) event produced by World Wrestling Entertainment (WWE). It was held for wrestlers from the promotion's Raw, SmackDown!, and ECW brand divisions. The event took place on June 3, 2007, at the Jacksonville Veterans Memorial Arena in Jacksonville, Florida. While the previous two years' events were held primarily as reunion shows for Extreme Championship Wrestling (ECW) alumni, the 2007 event was promoted as a WWE event with wrestlers from all three brands participating as following WrestleMania 23, brand-exclusive PPVs were discontinued. It was the final event to announce each individual brand as co-promoters of the PPV during the introductory graphics.

The main match on the Raw brand was John Cena versus The Great Khali in a Pinfalls Count Anywhere match for the WWE Championship. Cena won the match and retained the WWE Championship after pinning Khali following an FU off a crane onto the arena floor. The featured match on the SmackDown! brand was Edge versus Batista in a Steel Cage match for the World Heavyweight Championship, which Edge won after escaping the cage.

The primary match on the ECW brand was Vince McMahon versus Bobby Lashley in a Street Fight for the ECW World Championship, which Lashley won by pinfall after performing a spear. The featured matches on the undercard included Rob Van Dam versus Randy Orton in a Stretcher match and a Ladder match for the World Tag Team Championship between The Hardys (Matt and Jeff) and The World's Greatest Tag Team (Shelton Benjamin and Charlie Haas).