Golgo 13: Queen Bee (ゴルゴ13〜QUEEN BEE〜) is an original video animation that was released in 1998. Tesshō Genda provides the voice of Golgo 13 in the Japanese version, while in the English version, he is voiced by John DiMaggio. The film is set during the 2000 U.S. presidential election cycle and focuses on assassin Golgo 13, who is hired by a Democratic Party campaign manager to stop a female drug lord from assassinating the Democratic presidential nominee. Golgo, however, finds the job too easy and seeks to discover the true connection.

Produced by Tezuka Productions in association with Filmlink International and Goodhill Vision, Golgo 13: Queen Bee was released on 21 May 1998 in Japan by BMG Victor, and on DVD in English-speaking regions in 2001 by Urban Vision Entertainment.