Doña Lupe is a 1985 short horror film written and directed by Guillermo del Toro. It is del Toro's ninth short film, though the first eight remain unreleased. Del Toro filmed Doña Lupe at 19 years of age; reviewers have noted that the film "feels like the work of an amateur artist getting to grips with his craft".

In 2008, Doña Lupe saw its first commercial release as part of the Cinema 16: World Short Films DVD collection. In the audio commentary, del Toro apologizes for the film's poor quality and recounts anecdotes from its troubled production.