2000 Mules is a debunked 2022 American conspiracist political film from right-wing political commentator Dinesh D'Souza. The film falsely claims unnamed nonprofit organizations supposedly associated with the Democratic Party paid "mules" to illegally collect and deposit ballots into drop boxes in the swing states of Arizona, Georgia, Michigan, Pennsylvania, and Wisconsin during the 2020 presidential election. D'Souza has a history of creating and spreading false conspiracy theories.

The Associated Press (AP) reported that the film relies on "faulty assumptions, anonymous accounts and improper analysis of cellphone location data" provided by conservative non-profit True the Vote. FactCheck.org found the film's "supposed evidence is speculative". National Public Radio (NPR) reported True the Vote "made multiple misleading or false claims about its [own] work". AP reported that the assertion that True the Vote identified 1,155 paid mules in Philadelphia alone was false. The film presented a single unverified anonymous witness who said she saw people picking up what she "assumed" were payments for ballot collection in Arizona; no evidence of such payments was presented in any of the other four states. The film characterizes the alleged operation as "ballot trafficking" with "stash houses", but presents no evidence that ballots were illegally collected to be deposited in drop boxes.

A companion book was set to be released in early September 2022 but was abruptly recalled amidst legal threats and edited for release late in October. In 2024, Salem Media Group partially settled a lawsuit by a Georgia man who had been falsely accused of depositing fraudulent ballots in a ballot box. As part of the settlement, Salem disavowed the film and the book, pulled them from distribution and apologized.