The Tree's Knees is a 1931 one-reel short subject featuring Bosko, part of the Looney Tunes series. It was released in August 1931 and is directed by Hugh Harman and Rudolf Ising, the last cartoon in the series to be directed by the two. Every Looney Tunes cartoon after this was directed by Hugh Harman until 1933, and every Merrie Melodies cartoon was directed by Rudolf Ising until the aforementioned year. It is also the last Bosko cartoon to not feature the main character's (Bosko's) name in the title. The short is also notable for the extensive use of footage from the earlier short Ain't Nature Grand! that it reuses, in particular a scene of Bosko happily and innocently pursuing a butterfly.

The film score was composed by Frank Marsales.