Winnie-the-Pooh: Blood and Honey 2 (stylised as Winnie-the-Pooh: Blood and Honey II) is a 2024 British independent slasher film directed by Rhys Frake-Waterfield and written by Matt Leslie. It is the second installment of The Twisted Childhood Universe (TCU) and a sequel to Winnie-the-Pooh: Blood and Honey (2023), which serves as a horror retelling of A. A. Milne and E. H. Shepard's Winnie-the-Pooh books. The film stars Scott Chambers as Christopher Robin, and Ryan Oliva as the titular character, with Tallulah Evans, Teresa Banham, Peter DeSouza-Feighoney, Alec Newman, and Simon Callow in supporting roles. It follows Pooh as he embarks on a murderous rampage through Christopher Robin's childhood town to seek revenge on him for revealing his existence to the world.

Following the commercial success of Blood and Honey, director Frake-Waterfield expressed interest in a sequel that, eventually, was greenlit in November 2022. The film utilizes metafictional and film within a film elements as Chambers, Oliva, and Eddy McKenzie replace original cast members Nikolai Leon, Craig David Dowsett, and Chris Cordell in the roles of Christopher Robin, Winnie-the-Pooh, and Piglet, respectively.

Winnie-the-Pooh: Blood and Honey 2 premiered in London on 18 March 2024, and was theatrically released in the United States on 26 March 2024. Many critics considered the film to be an improvement over its predecessor. It grossed $7.5 million worldwide on a budget of $500,000. A sequel, Winnie-the-Pooh: Blood and Honey 3, is scheduled to be released in 2026.