Winnie-the-Pooh: Blood and Honey 2 (stylised as Winnie-the-Pooh: Blood and Honey II) is a 2024 British independent slasher film directed by Rhys Frake-Waterfield and written by Matt Leslie. It is the second installment of The Twisted Childhood Universe (TCU) and a sequel to Winnie-the-Pooh: Blood and Honey (2023). Like its predecessor, it is a horror parody of A. A. Milne and E. H. Shepard's Winnie-the-Pooh books. The film stars Scott Chambers as Christopher Robin, and Ryan Oliva as the titular character, with Tallulah Evans, Teresa Banham, Peter DeSouza-Feighoney, Alec Newman, and Simon Callow in supporting roles. It follows Pooh as he embarks on a murderous rampage through Christopher Robin's childhood town to seek revenge on him for revealing his existence to the world.

Following the commercial success of Blood and Honey, director Frake-Waterfield expressed interest in a sequel that, eventually, was greenlit in November 2022. The film utilizes metafictional and film within a film elements as Chambers, Oliva, and Eddy McKenzie replace original cast members Nikolai Leon, Craig David Dowsett, and Chris Cordell in the roles of Christopher Robin, Winnie-the-Pooh, and Piglet, respectively.

Winnie-the-Pooh: Blood and Honey 2 premiered in London on 18 March 2024, and was theatrically released in the United States on 26 March 2024. It grossed $7.5 million worldwide on a budget of $500,000. A sequel, Winnie-the-Pooh: Blood and Honey 3, is scheduled to be released in 2026.