Twice Upon a Time is a 1953 British comedy film directed by Emeric Pressburger and starring Hugh Williams, Elizabeth Allan, Yolande Larthe, and Charmian Larthe. It is based on the 1949 novel Lisa and Lottie by Erich Kästner. It concerns twin sisters who are separated when their parents divorce. They meet again by accident when they are sent to the same summer camp, and they hatch a plan to reunite their parents.

Lotte and Lisa had already been adapted into the films Two Times Lotte (1950) and Hibari no komoriuta (1951). Twice Upon a Time was the first English-language film adaptation; the story was later adapted as The Parent Trap (1961) and has been remade a number of times in English and many other languages. It was shot at Shepperton Studios with sets designed by the art director Arthur Lawson (designer).

The film is the only solo directing credit for Pressburger, whose other directing credits are in association with Michael Powell.