My Little Pony: Make Your Mark is a Canadian animated television series tied to the fifth incarnation (also referred to as the fifth generation or "G5") of Hasbro's My Little Pony toyline. Set after the events of the film My Little Pony: A New Generation (2021), the series follows five ponies—Sunny Starscout (Jenna Warren), an idealistic earth pony; Izzy Moonbow (Ana Sani), a creative unicorn; Hitch Trailblazer (J.J. Gerber), a responsible earth pony; and sisters Zipp Storm (Maitreyi Ramakrishnan) and Pipp Petals (AJ Bridel), two pegasi princesses—on their adventures and battles across Equestria. The ponies are joined later on by Misty Brightdawn, a shy unicorn and former associate of the series' antagonist, the villainous alicorn Opaline Arcana.

Developed by Gillian Berrow for Netflix, the 44-minute special episode Make Your Mark premiered as a teaser on May 26, 2022, followed by the release of eight further episodes on September 26. The series is produced by Entertainment One with animation provided by Atomic Cartoons, and a total of four 44-minute special episodes and twenty-three 22-minute episodes have been ordered.

The sixth and final chapter was released on November 23, 2023.