An American Tail: The Treasure of Manhattan Island (also known as An American Tail 3: The Treasure of Manhattan Island) is a 1998 American animated adventure film produced by Universal Cartoon Studios and directed by Larry Latham. It is the third film in the An American Tail series, the first to be released direct-to-video, and the first in the series to use digital ink and paint.

While retaining Dom DeLuise throughout the film series, the film reunites the voice cast of Nehemiah Persoff and Erica Yohn from both An American Tail (1986) and An American Tail: Fievel Goes West, Pat Musick from An American Tail with the return of her character Tony Toponi, and introduces Elaine Bilstad, René Auberjonois, David Carradine, John Kassir, Ron Perlman, Tony Jay and Richard Karron. Fievel and Tanya Mousekewitz's voices were performed by Thomas Dekker and Lacey Chabert, replacing the previous voice actors Phillip Glasser, Amy Green from the 1986 film, and Cathy Cavadini from both Fievel Goes West and the TV series Fievel's American Tails.

The film premiered in the United Kingdom on November 16, 1998, and was released in the United States and Canada on February 15, 2000. The film explores the themes of labor exploitation, worker's rights, xenophobia and the effects of European colonialism.

This was Erica Yohn and Elaine Bilstad's final film appearances.