The Lone Rider Ambushed is a 1941 American western film directed by Sam Newfield and written by Oliver Drake. The film stars George Houston as the Lone Rider and Al St. John as his sidekick "Fuzzy" Jones, with Maxine Leslie, Frank Hagney, Jack Ingram and Hal Price. The film was released on August 29, 1941, by Producers Releasing Corporation.

This is the fifth movie in the Lone Rider series, which spans seventeen films—eleven starring George Houston, and a further six starring Robert Livingston. In this film, Houston plays a dual role as both the Lone Rider and the villain, Keno Harris.

Houston, once an opera singer, sang three songs in this film: "Without You Darling, Life Wouldn't Be the Same", "Ridin' Roamin' on the Prairie" and "If It Hadn't Been for You". The songs were written by Johnny Lange and Lew Porter.

This film was later released on DVD as Trapped in the Badlands.