The Lone Rider Crosses the Rio is a 1941 American Western film directed by Sam Newfield and written by William Lively. The film stars George Houston as the Lone Rider and Al St. John as his sidekick "Fuzzy" Jones, with Roquell Verria, Charles King, Julian Rivero and Stephen Chase. The film was released on February 28, 1941, by Producers Releasing Corporation.

This is the second movie in the Lone Rider series, which spans 17 films—eleven starring George Houston, and a further six starring Robert Livingston.

Houston, once an opera singer, sang three songs in this film: "It's a Gay Fiesta", "Git Along Cowboy" and "I'm Pancho, the Mexican Bandit". The songs were written by Johnny Lange and Lew Porter.