Scooby-Doo! and the Legend of the Vampire is a 2003 American direct-to-video animated adventure film, and the fifth in a series of direct-to-video films based upon the Scooby-Doo Saturday-morning cartoons. It was completed in 2002, and released on March 4, 2003, and it was produced by Warner Bros. Animation, but included a copyright for Hanna-Barbera Cartoons, Inc..

It is the first Scooby-Doo direct-to-video film to have the flatter, bright art style and visuals of the What's New, Scooby-Doo? series, departing from the darker shading and effects used in the four prior released films, the first to return to the original format where the monster is not real and the first to return to a lighter tone than that of the prior and darker Scooby made-for-video films.

This film served as Joseph Barbera's first solo animated production effort without longtime partner William Hanna, who died in 2001, and is one of two direct-to-video films to reunite the 1969–73 voice cast for the human Mystery Inc. members consisting of Frank Welker, Casey Kasem, Nicole Jaffe, and Heather North. Since Don Messick died in 1997, Welker provides Scooby's voice (in addition to voicing Fred Jones).