Assassin's Creed is a 2016 historical science fiction action film based on the video game franchise published by Ubisoft. The film is directed by Justin Kurzel, written by Michael Lesslie, Adam Cooper and Bill Collage, and stars Michael Fassbender (who also produced), as well as Marion Cotillard, Jeremy Irons, Brendan Gleeson, Charlotte Rampling and Michael K. Williams. The film is set in the same universe as the video games but features an original story that expands the series' mythology. The plot revolves around Callum "Cal" Lynch (Fassbender), who is abducted by the Abstergo Foundation because of his heritage. Cal's ancestor, Aguilar de Nerha, was a member of the Assassin Brotherhood—a fictional organization inspired by the real-life Order of Assassins—active during the Spanish Inquisition in the late 15th-century, who swore to protect the Apple of Eden, an artifact believed to contain the key to humanity's free will. Cal must accept his Assassin heritage and stop Abstergo, the Templar Order of the modern-day, from finding the Apple and using it to enslave humanity.

Filming began in late August 2015 and concluded in January 2016. Assassin's Creed was released by 20th Century Fox on December 21, 2016, in the United States and France. It generally received negative reviews from critics that were primarily aimed towards the plot and writing, though some considered it an improvement over previous video game film adaptations. The film underperformed at the box office, grossing $240.7 million worldwide against its $125 million budget. A sequel was planned, but due to the film's negative reception and disappointing box office result, it was cancelled by Disney after its acquisition of 21st Century Fox in 2019.