009-1 is a Japanese manga series written and illustrated by Shotaro Ishinomori. The manga was serialized in the Futabasha publication Weekly Manga Action from 1967 to 1970, then returned briefly in 1974. The story concerns Miléne Hoffman ("Mylene" in the English translation), a female cyborg who works as a secret agent. The Japanese title of the manga was 009ノ1, or "Zero Zero Ku-no-ichi", a pun on kunoichi (female ninja) and a reference to the main character's espionage occupation.

The original manga was adapted into a live-action drama for Fuji Television in 1969 entitled Flower Action 009ノ1. The manga was also adapted into a 12-episode anime series by Ishimori Entertainment and first broadcast on TBS TV in Japan in late 2006. In June 2013, it was announced the manga would be adapted into a live-action film entitled 009-1: The End of the Beginning to be directed by Koichi Sakamoto, and starring Mayuko Iwasa. Minehiro Kinomoto, Nao Nagasawa, Mao Ichimichi, Shizuka Midorikawa, Naoto Takenaka, and Aya Sugimoto. It premiered on September 7, 2013.

Although it was also created by Ishinomori, and features similar themes, this seinen manga, despite the "00" name and the cybernetized protagonists, has no relation to his previous work Cyborg 009, a shōnen manga (although in the original manga, the cyborgs from Cyborg 009 actually make appearances in some chapters). In the final episode, there are two homages to Gerry Anderson television shows involving the Moon, an Eagle Transport from Space: 1999 and SHADO Mobiles from UFO. In the episode "Reverse-Explosion" a spaceship is destroyed by impacting the Moon. This is Thunderbird 5 from Thunderbirds.