Late Night with Jimmy Fallon is an American television talk show broadcast by NBC. The show was the third installment of the Late Night franchise. Hosted by Jimmy Fallon, it aired from March 2, 2009, to February 7, 2014, replacing Late Night with Conan O'Brien and was in turn replaced by Late Night with Seth Meyers. The show aired weeknights at 12:35 a.m. Eastern/11:35 p.m. Central, on NBC.

The third incarnation of the Late Night franchise originated by David Letterman, the program originated from NBC Studio 6B (and Studio 6A for the final six months of its run) in the Comcast Building at 30 Rockefeller Center in New York City. The show typically opened with a brief monologue from Fallon, followed by a comedy "desk piece," as well as prerecorded segments and audience competitions. The next segment was devoted to a celebrity interview, with guests ranging from actors and musicians to media personalities and political figures. Hip hop/neo soul band The Roots served as the show's house band, and Saturday Night Live writer and producer Steve Higgins was the show's announcer. The show then closed with either a musical or comedy performance, or a cooking segment. The show frequently employed digital media into its comedy, which became crucial to its success.

In 2013, Fallon was selected by NBC to succeed a retiring Jay Leno as host of The Tonight Show. The final episode of Late Night under Fallon aired one night after Leno's final episode of The Tonight Show on February 6, 2014. Most of the cast and crew immediately began working on The Tonight Show Starring Jimmy Fallon, which premiered on February 17, 2014.

Seth Meyers was named as Fallon's replacement, and Late Night with Seth Meyers debuted after the Sochi Olympics.

The show's ratings remained above its rival shows throughout most of the series' run. In 2011, the show garnered two Emmy Award nominations, including Outstanding Variety, Music or Comedy Series.