My-Otome (舞-乙HiME, Mai-Otome) is an anime series created by Sunrise. Directed by Masakazu Obara and written by Hiroyuki Yoshino, it is a spinoff of the My-HiME anime series and as such My-Otome takes place in a new setting with new main characters.

The series originally premiered on TV Tokyo from October 6, 2005 to March 30, 2006. An OVA sequel to the series, My-Otome Zwei, has been made and released in Japan, and a prequel, My-Otome 0: S.ifr, has been completed. The series was licensed for North American distribution by Bandai Entertainment. Zwei leaves the story open for future sequels, and Sifr for future prequels. At Otakon 2013, Funimation Entertainment had announced that they have rescued My-Otome, along with a handful of other former BEI titles. They also announced at the 2017 New York Comic Con that they will release My-HiMe, My-Otome, and the My-Otome Zwei + My-Otome 0: S.ifr pack, all as Blu-Ray + DVD combo packs on January 8, 2018. They will start pre-orders on October 15, 2017.

A new series was announced in June 2008, later announced as the My-HiME EXA manga.