Gotham Girls is an American Flash animated web series focusing on several of the female characters of Gotham City, produced jointly by Warner Bros. Animation and Noodle Soup Productions. The series, which ran from 2000 to 2002, starred Harley Quinn, Poison Ivy, Batgirl, Catwoman, Renee Montoya and Zatanna in short stories of varying length about the daily lives of the characters. It takes place in the DC Animated Universe, with Arleen Sorkin, Diane Pershing, Adrienne Barbeau, Tara Strong, and Bob Hastings reprising their roles from Batman the Animated Series and The New Batman Adventures.

It is also the name of a related comic book series.